Isolation and partial characterization of multiple DNA polymerases of the murine myeloma, MOPC-21.

An understanding of the functions of DNA-synthesizing enzymes in a tumor cell would be helpful in developing our concepts of the molecular basis of cancer. The availability of homogeneous populations of myeloma cells provides a well-characterized source of such enzymes in a tumor line that maintains a high degree of differentiated function. We have previously reported in preliminary form the 1st description of the multiple DNA polymerases of the myeloma line MOPC-21 (10). We report herewith an efficient technique for the fractionation of these activities and a description of some of the properties of these enzymes.